Calpain-1 Substrate II; Fluorogenic

Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetCalpain-1 Substrate

General description

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively.

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively. Excitation: ~335 nm; emission: ~505 nm.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cuerrier, D., et al. 2007. J. Biol. Chem.282, 9600. Cuerrier, D., et al. 2005. J. Biol. Chem.280, 40632.

Packaging

Packaged under inert gas

1 mg in Glass bottle

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

H₂N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO₂H

Warning

Toxicity: Irritant (B)